A simple and efficient method for extraction of Taq DNA polymerase
نویسندگان
چکیده
منابع مشابه
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Three DNA polymerases, namely E.coli DNA polymerase 1 (Klenow), reverse transcriptase and T7 DNA polymerase (sequenase), are commonly used for DNA sequencing by the chain termination method of Sanger and colleagues [1). However, the secondary structure of the DNA template can impede the progress of all three polymerases. I have developed a novel procedure in which the thermostable polymerase of...
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Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the T...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2015
ISSN: 0717-3458
DOI: 10.1016/j.ejbt.2015.08.001